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a , time-lapse projected intensity image of 300-nm diameter nanoparticles suspended in water (scale bar 5 μm ). The time-lapse intensity images were reconstructed from acquired interferograms at a single plane over 10 seconds. The coverslip of petri-dish was cut to open a small slit to trigger the flowing of nanoparticles in solutions. b , measured speed distributions of nanoparticles in both water and 10% w/w glycerol solutions by using the same petri-dish. c , volumetric rendering view of multi-cellular systems <t>(MDA-MB-435S)</t> with field of view 90 μm × 90 μm × 23 μm along xyz dimensions. d-e , reconstructed intensity (left at d and top at e) and phase (right at d and bottom at e) images of cells at different depths. The intensity images at middle and right columns of e were gamma-corrected by a factor of 0.5 to improve image contrast. Scale bars are 10 μm .
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a , time-lapse projected intensity image of 300-nm diameter nanoparticles suspended in water (scale bar 5 μm ). The time-lapse intensity images were reconstructed from acquired interferograms at a single plane over 10 seconds. The coverslip of petri-dish was cut to open a small slit to trigger the flowing of nanoparticles in solutions. b , measured speed distributions of nanoparticles in both water and 10% w/w glycerol solutions by using the same petri-dish. c , volumetric rendering view of multi-cellular systems <t>(MDA-MB-435S)</t> with field of view 90 μm × 90 μm × 23 μm along xyz dimensions. d-e , reconstructed intensity (left at d and top at e) and phase (right at d and bottom at e) images of cells at different depths. The intensity images at middle and right columns of e were gamma-corrected by a factor of 0.5 to improve image contrast. Scale bars are 10 μm .
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a , time-lapse projected intensity image of 300-nm diameter nanoparticles suspended in water (scale bar 5 μm ). The time-lapse intensity images were reconstructed from acquired interferograms at a single plane over 10 seconds. The coverslip of petri-dish was cut to open a small slit to trigger the flowing of nanoparticles in solutions. b , measured speed distributions of nanoparticles in both water and 10% w/w glycerol solutions by using the same petri-dish. c , volumetric rendering view of multi-cellular systems <t>(MDA-MB-435S)</t> with field of view 90 μm × 90 μm × 23 μm along xyz dimensions. d-e , reconstructed intensity (left at d and top at e) and phase (right at d and bottom at e) images of cells at different depths. The intensity images at middle and right columns of e were gamma-corrected by a factor of 0.5 to improve image contrast. Scale bars are 10 μm .
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a , time-lapse projected intensity image of 300-nm diameter nanoparticles suspended in water (scale bar 5 μm ). The time-lapse intensity images were reconstructed from acquired interferograms at a single plane over 10 seconds. The coverslip of petri-dish was cut to open a small slit to trigger the flowing of nanoparticles in solutions. b , measured speed distributions of nanoparticles in both water and 10% w/w glycerol solutions by using the same petri-dish. c , volumetric rendering view of multi-cellular systems <t>(MDA-MB-435S)</t> with field of view 90 μm × 90 μm × 23 μm along xyz dimensions. d-e , reconstructed intensity (left at d and top at e) and phase (right at d and bottom at e) images of cells at different depths. The intensity images at middle and right columns of e were gamma-corrected by a factor of 0.5 to improve image contrast. Scale bars are 10 μm .
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a , time-lapse projected intensity image of 300-nm diameter nanoparticles suspended in water (scale bar 5 μm ). The time-lapse intensity images were reconstructed from acquired interferograms at a single plane over 10 seconds. The coverslip of petri-dish was cut to open a small slit to trigger the flowing of nanoparticles in solutions. b , measured speed distributions of nanoparticles in both water and 10% w/w glycerol solutions by using the same petri-dish. c , volumetric rendering view of multi-cellular systems <t>(MDA-MB-435S)</t> with field of view 90 μm × 90 μm × 23 μm along xyz dimensions. d-e , reconstructed intensity (left at d and top at e) and phase (right at d and bottom at e) images of cells at different depths. The intensity images at middle and right columns of e were gamma-corrected by a factor of 0.5 to improve image contrast. Scale bars are 10 μm .
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a , time-lapse projected intensity image of 300-nm diameter nanoparticles suspended in water (scale bar 5 μm ). The time-lapse intensity images were reconstructed from acquired interferograms at a single plane over 10 seconds. The coverslip of petri-dish was cut to open a small slit to trigger the flowing of nanoparticles in solutions. b , measured speed distributions of nanoparticles in both water and 10% w/w glycerol solutions by using the same petri-dish. c , volumetric rendering view of multi-cellular systems <t>(MDA-MB-435S)</t> with field of view 90 μm × 90 μm × 23 μm along xyz dimensions. d-e , reconstructed intensity (left at d and top at e) and phase (right at d and bottom at e) images of cells at different depths. The intensity images at middle and right columns of e were gamma-corrected by a factor of 0.5 to improve image contrast. Scale bars are 10 μm .
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a , time-lapse projected intensity image of 300-nm diameter nanoparticles suspended in water (scale bar 5 μm ). The time-lapse intensity images were reconstructed from acquired interferograms at a single plane over 10 seconds. The coverslip of petri-dish was cut to open a small slit to trigger the flowing of nanoparticles in solutions. b , measured speed distributions of nanoparticles in both water and 10% w/w glycerol solutions by using the same petri-dish. c , volumetric rendering view of multi-cellular systems <t>(MDA-MB-435S)</t> with field of view 90 μm × 90 μm × 23 μm along xyz dimensions. d-e , reconstructed intensity (left at d and top at e) and phase (right at d and bottom at e) images of cells at different depths. The intensity images at middle and right columns of e were gamma-corrected by a factor of 0.5 to improve image contrast. Scale bars are 10 μm .
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a , time-lapse projected intensity image of 300-nm diameter nanoparticles suspended in water (scale bar 5 μm ). The time-lapse intensity images were reconstructed from acquired interferograms at a single plane over 10 seconds. The coverslip of petri-dish was cut to open a small slit to trigger the flowing of nanoparticles in solutions. b , measured speed distributions of nanoparticles in both water and 10% w/w glycerol solutions by using the same petri-dish. c , volumetric rendering view of multi-cellular systems (MDA-MB-435S) with field of view 90 μm × 90 μm × 23 μm along xyz dimensions. d-e , reconstructed intensity (left at d and top at e) and phase (right at d and bottom at e) images of cells at different depths. The intensity images at middle and right columns of e were gamma-corrected by a factor of 0.5 to improve image contrast. Scale bars are 10 μm .

Journal: bioRxiv

Article Title: Scanless temporal focusing enables high-speed three-dimensional quantitative phase microscopy

doi: 10.64898/2026.03.04.709629

Figure Lengend Snippet: a , time-lapse projected intensity image of 300-nm diameter nanoparticles suspended in water (scale bar 5 μm ). The time-lapse intensity images were reconstructed from acquired interferograms at a single plane over 10 seconds. The coverslip of petri-dish was cut to open a small slit to trigger the flowing of nanoparticles in solutions. b , measured speed distributions of nanoparticles in both water and 10% w/w glycerol solutions by using the same petri-dish. c , volumetric rendering view of multi-cellular systems (MDA-MB-435S) with field of view 90 μm × 90 μm × 23 μm along xyz dimensions. d-e , reconstructed intensity (left at d and top at e) and phase (right at d and bottom at e) images of cells at different depths. The intensity images at middle and right columns of e were gamma-corrected by a factor of 0.5 to improve image contrast. Scale bars are 10 μm .

Article Snippet: MDA-MB-435S cells were cultured under aseptic conditions in Dulbecco’s Modified Eagle Medium (DMEM; ATCC, 30-2002) containing 4 mM L-glutamine, 4500 mg/L glucose, 1 mM sodium pyruvate, and 1500 mg/L sodium bicarbonate, supplemented with 10% fetal bovine serum (FBS; ATCC, 30-2021).

Techniques: